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发明公开
CN103382510A 检测口蹄疫A型病毒的荧光RT-PCR引物和探针及其试剂盒 无效 - 驳回

检测口蹄疫A型病毒的荧光RT-PCR引物和探针及其试剂盒
摘要：
本发明公开了检测口蹄疫A型病毒的荧光RT-PCR引物和探针及其试剂盒，所述引物的上游引物核苷酸序列为5′-TGCCCACTTCCTTCAAC-3′，下游引物核苷酸序列为5′-CTCCAGGTCAGAGAAGTAGT-3′；所述探针为5′-F-ATGTCATTGACCTCATGCAGACCCACCA-Q-3′，其中F为报告荧光基团，Q为淬灭荧光基团；所述试剂盒包括所述的引物和探针。采用本发明检测口蹄疫A型病毒，具有敏感性高、特异性强、自动化程度高、快速简便等优点。
摘要（英）：

The invention discloses a fluorescent RT-PCR primer and probe for detecting an A-type foot and mouth disease virus and a kit of the primer and the probe. An upstream primer nucleotide sequence of the primer is 5'-TGCCCACTTCCTTCAAC-3', and a downstream primer nucleotide sequence is 5'-CTCCAGGTCAGAGAAGTAGT-3'; the probe is 5'-F-ATGTCATTGACCTCATGCAGACCCACCA-Q-3', wherein F is a reporter fluorescent group, and Q is a quencher fluorescent group; the kit comprises the primer and the probe. The primer, the probe and the kit are used for detecting the A-type foot and mouth disease virus and have the advantages of being high in susceptibility, high in specificity, high in automation degree, fast, simple and the like.

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