The invention relates to a separation method for single-walled carbon nano-tubes with special chirality. According to the separation method, interaction of an organic solvent and ultrasonic wave is adopted to separate tube bundles of single-walled carbon nano-tubes into the single carbon nano-tube, centrifugation is performed to separate the carbon nano-tubes dispersed in the solvent from the precipitate to obtain a single-walled carbon nano-tube solution, an organic amine is added to the solution, the organic amine and the dispersed single carbon nano-tube are subjected to absorption through an ultrasonic effect, the metallic single-walled carbon nano-tubes have strong amine absorption ability, and are stable in the solution, high-speed centrifugation is performed to remove the semiconducting carbon nano-tubes so as to obtain a metallic carbon nano-tube solution, a 0.22 mum microporous filtration membrane is adopted to filter the supernatant, and ethanol is adopted to wash the organic amine absorbed on the carbon nano-tubes to obtain the metallic carbon tubes. According to the present invention, the ultrasound-centrifugation process is only required to be repeated twice to obtain the metallic carbon tubes so as to break through the bottleneck of the original separation method, achieve a purpose of large-scale separation of metallic carbon tubes, and achieve the magnitude of g or more.