The invention discloses a cut stem fumet for tobacco as well as a preparation method and application thereof. The preparation method comprises the following steps: performing Maillard reaction on maple sugar serving as a natural sugar source and mixed amino acid, wherein the mass ratio of the maple sugar to the mixed amino acid is (10-25):1; additionally adding a small amount of ammonia water or a specific aldehyde substance into the reaction mixture; and adjusting the pH value to be 7 to 10, controlling the temperature to be 90 to 120 DEG C and reacting for 3 to 6 hours under the stirring condition to obtain the cut stem fumet for tobacco. The cut stem fumet for tobacco is applied to cut stem perfuming and the adding ratio of the cut stem fumet for tobacco is 0.03 to 0.08% of the weight of cut stems, so tobacco flavor can be improved obviously, wood mixed gas of the cut stems can be covered, and dry feeling of an oral cavity is reduced.