The invention relates to a self-adaptive tracking method for a direct sequence spread spectrum signal in a real-time positioning system. In the method, a phase difference between a local code and a received code is estimated according to a correlation value of an advance, a current and a lagged channels in a delay locked loop; and then based on an estimated phase difference, a local code phase in a tracking process is adjusted to be within a scope of tracking accuracy. According to the invention, the advantages are that: while the direct sequence spread spectrum signal tracking accuracy is guaranteed, the signal synchronization time is effectively shortened; and the real-time positioning system performance is improved.