The invention relates to an analyzing method for continuous cropping peanut soil eukaryotic microbial community succession. The method includes adopting a pot experiment, selecting soil free of planting peanuts, mixing the soil complete uniformly and dividing to pots, and planting the peanuts for three years continuously. Times for sowing and harvesting in the three year are consistent. In each planting period, soil at peanut roots are collected in the period of seedling and the period of harvesting, soil genomic DNA are extracted, and 18SrRNA gene libraries are built. By analyzing and comparing the built libraries, laws of the continuous cropping peanut soil eukaryotic microbial community succession can be acquired accordingly. By the aid of the analyzing method, community structure and dominant communities of continuous cropping peanut soil eukaryotic microbial community can be definite, and the succession laws in the process of peanut continuous cropping of each eukaryotic microbial community can be acquired.