The invention discloses a method for seed germination and seedling culture of tutcheria microcarpa. The method comprises the following steps: (1) collecting fresh and ripe fruits and covering the fruits by using a wet tissue with the relative humidity of 70%-90%; placing the fruits into a closed container and carrying out after-ripening treatment under an indoor dark condition; then shucking to obtain seeds; immersing the seeds by using a potassium permanganate solution with the mass percentage of 0.1%-0.3%; and drying in the shade for future use; and (2) spraying a fenaminosulf solution which is diluted for 2,000-3,000 times on a base material and standing for three days; watering clean water thoroughly on the fourth day; scattering the immersed seeds into the base material and covering the base material with a layer of a shading net; and watering or spraying every day for preserving moisture, wherein the base material comprises the following components in mass ratio of ceramsite to perlite to vermiculite to river sand to peat soil being 1 to 2 to 2 to 2 to 4 and the seed germination temperature is 10-29 DEG C. According to the method, the emergence rate of the tutcheria microcarpa is improved through the after-ripening treatment and dormancy breaking, so that the seedling culture needed by artificial cultivation is achieved and artificial cultivation breeding can be carried out by seeding.