A manufacturing method of an adult cat musclespecimen comprises the following steps: the corpse of an adult cat is selected for anesthesia within five minutes after the adult cat is put into death; an opening with the diameter of 0.6 to 1 cm is cut on the skin of an abdominal cavity of the corpse of the cat by using a scalpel so as to expose a heart, and polyethylene glycol and water are adopted for graded perfusion to the heart; then the skin of the adult cat is peeled off, and the whole muscle of the cat is separated out entirely; after being degreased by adopting a mixed solution of absolute ethyl alcohol and xylene with the ratio of 2 to 1, and the whole muscle of the cat is aired for 5 to 7 days at a dark and ventilation part, then is soaked into diluted hydrogen peroxide, taken out after being soaked for 2 to 5 days, cleaned by clear water, and aired; then the muscle is fixed on a glass plate by using glue so as to enable the muscle to hold a standing posture of the cat, and then is put into a specimen jar, that is a full body adult cat musclespecimen is prepared. The method provided by the invention is simple, the cost is low, the prepared adult cat musclespecimen is easy to keep, the structure is complete and beautiful, and the shape and the position are clear and nature.