The invention relates to a detection method of residual quantity of acrylamide in anion/non-ionic type PAM. The method comprises the following steps: (1) preparation of extract liquor: dissolving 0.1 Kg/L polyacrylamide in a methyl alcohol solvent with the volume concentration of 75-82%, utilizing a supersonic cleaner for processing the solution for one hour in an ultrasonic manner, standing, pouring out the supernatant liquid, thinning up the supernatant liquid to a volume ten times of the original volume, and using an aqueous phase filter head with the length of 0.22 micron for filtering so as to obtain the extract liquor; (2) analysis of high performance liquid chromatography, wherein the chromatographic column is a Huijie SinoChromODS-BPC-18 reversed-phase column, the moving phase comprises A referring to the 10 mmol/LKH2PO4 solution and B referring to methyl alcohol, and the volume ratio of A to B is 85:15; the detection wavelength is 210 nm; the flow rate is 0.8 ml/min; the column temperature is the room temperature; the sample volume is 20 Mul. According to the invention, the pretreatment time is decreased from 24 hours to 1 hour so as to meet the rapid detection requirement of an enterprise on a sample.