The invention relates to a bacterial cellulose-chitosan plural gel as well as a preparation method thereof and application thereof in intracorporal treatment. The plural gel consists of a chitosan solution, bacterial cellulose, an antibacterial agent, a thickening agent solution and a neutralizing agent. The bacterial cellulose-chitosan plural gel disclosed by the invention is applied to the preparation of hemorrhoid medicaments and the preparation of gynecological vaginitis and cervicitis medicaments. The preparation method of the plural gel comprises the following steps of: uniformly mixing the chitosan solution, the thickening agent solution, an antibacterial agent solution and the bacterial cellulose, then standing, and finally defoaming. The main raw material namely chitosan of the plural gel is rich in resource, and the bacterial cellulose-chitosan plural gel overcomes the defects of poor mechanical properties and unstable antibacterial effect of the conventional polymer gel.