The invention provides a control method suitable for multiple-seat 500kV transformer substation remote-control transmission. The method includes the following steps that before a scheduling main station and a monitoring center main station test and remotely control a remote-controlled object, the remote-control terminal on a back plate of a testing and controlling device where the object exists is unplugged, the scheduling main station is used for carrying out remote-control transmission on the remote-controlled object, an access shift of a multimeter for the end of a 500kV transformer substation is used for testing on-off of a remote-control connection point and consistency and accuracy of a remote-control test are verified; a QK handle on a breaker testing and controlling screen and a testing and controlling protection integrated screen of the 500kV transformer substation is switched to the ground position by breaking remote control outlet pressing plates of all testing and controlling devices of the 500kV transformer substation so that safe and stable operation of a power grid is ensured and the accident of the power grid is avoided. The control method suitable for multiple-seat 500kV transformer substation remote-control transmission has the advantages that under the on-line condition, tests on the remote-control transmission function of six 500kV transformer substations can be completed within 30 days, the working efficiency is improved, black-out transmission time is shortened and technical guarantee is provided for safe and stable implementation of the whole main power grid.