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发明公开
CN103484538A 检测肠球菌耐药性的组合物及方法 失效 - 权利终止

检测肠球菌耐药性的组合物及方法
摘要：
本发明涉及用于检测肠球菌耐药性的组合物和方法，其特征在于利用一对或多对特异性引物和多重PCR方法对肠球菌耐药性进行检测，其中组合物包含以下寡核苷酸引物对：肠球菌16s RNA特异性引物对：SEQ ID No.1和SEQ ID No.2；以及以下耐药基因特异性引物对中的一对或多对：aac-aph特异性引物对：SEQ ID No.3和SEQ ID No.4，tetM特异性引物对：SEQ ID No.5和SEQ ID No.6，ermB特异性引物对：SEQ ID No.7和SEQ ID No.8，TEM特异性引物对：SEQ ID No.9和SEQ ID No.10以及aad特异性引物对：SEQ ID No.11和SEQ ID No.12。使用本发明，能够简便、快速、灵敏度高且特异性强地测定食品中肠球菌的耐药性。
摘要（英）：

The invention relates to a composition and a method for detecting drug resistance of enterococci. The composition and the method are characterized in that the drug resistance of the enterococci is detected by using one pair or multiple pairs of specific primers and a multiple polymerase chain reaction (PCR) method, wherein the composition contains the following oligonucleotide primer pairs: an enterococci 16s RNA (ribonucleic acid) specific primer pair: SEQ ID No. 1 and SEQ ID No. 2, and one or more of the following drug resistant gene specific primer pairs: an aac-aph specific primer pair: SEQ ID No. 3 and SEQ ID No. 4, a tetM specific primer pair: SEQ ID No. 5 and SEQ ID No. 6, an ermB specific primer pair: SEQ ID No. 7 and SEQ ID No. 8, a TEM specific primer pair: SEQ ID No. 9 and SEQ ID No. 10 and an aad specific primer pair: SEQ ID No. 11 and SEQ ID No. 12. By using the composition and the method, the drug resistance of the enterococci in food can be simply and quickly measured with high sensitivity and strong specificity.

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