The invention discloses a manufacturing method of a pair of ethylene-propylene-diene monomer rubber insulating gloves for a hot-line work of 35kV. The method comprises the following steps: adding stearic acid, zinc oxide, 2-mercapto benzimidazole, carbon black and zinc methacrylate into ethylene-propylene-diene monomer rubber to be mixed; adding N-annular caproyl-2-truxene thiazole sulfamide, tetramethyl thiuram disulfide, dicumyl peroxide and sulphur when the mixing comes to an end so as to obtain a rubber material; placing the rubber material which is mixed well and shearing into a rubber slice; putting the rubber slice into a mould; vulcanizing the mould on a vulcanizing machine; and cooling and drying the mould, thereby obtaining the pair of ethylene-propylene-diene monomer rubber insulating gloves for the hot-line work of 35kV. The pair of ethylene-propylene-diene monomer rubber insulating gloves cannot be broken through because being capable of bearing the power frequency withstand voltage of 50kV for one minute, and can meet the requirement of the hot-line work of 35kV even suffering the leakage current below 10mA. In addition, the usage flexibility and the working efficiency of operating personnel are not influenced.