The invention relates to a connecting and sealing structure for a valve cover and a valve rod of a stop valve. The connecting and sealing structure comprises a valve body, the valve rod and the valve cover, wherein the valve body is provided with an inlet and an outlet, the valve cover is connected with the valve body, the valve rod is connected with the valve cover, a hand shank is arranged at the upper end of the valve rod, and a seal cap is arranged at the lower end of the valve rod and extends into the valve body. The portion, close to the hand shank, of the valve rod is connected with the upper end of the valve cover through threads, the lower end of the valve cover is connected with the upper surface of the valve body, a packing hole is formed in the center of the valve cover, the middle of the valve rod passes through the packing hole, and the portion between the valve rod and the wall of the packing hole is filled with sealing packing. A gland is arranged on the packing hole, and the gland is fixedly connected with the valve cover through screws. In comparison with the prior art, the packing hole in the center of the valve cover is filled with the sealing packing, the gland is arranged on the packing hole and fixedly connected with the valve cover through the screws, and the seal cap is matched with a through hole and forms seal connection with the through hole. The connecting and sealing structure is compact in structure, high in strength, good in seal, long in service life and convenient to operate.