The invention belongs to the field of pesticides, and particularly relates to an agricultural sterilization composition with fenaminstrobin and isoprothiolane as active ingredients. The composition is mainly used for preventing and treating fungal diseases such as rice blast, and meanwhile has the characteristics that the crop quality is improved and the crop yield is increased. The composition has more acting mechanisms and sites, can effectively delay the occurrence and the development of drug resistance of pathogens, is simple in preparation process, is low in cost and has an obvious economic benefit.