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发明公开
CN103529181A 牛奶制品中青霉素酶的AlphaLISA检测试剂盒 无效 - 驳回

牛奶制品中青霉素酶的AlphaLISA检测试剂盒
摘要：
本发明提供了一种牛奶制品中青霉素酶的AlphaLISA检测试剂盒，其包括青霉素酶标准品、生物素化青霉素酶、抗青霉素酶单克隆抗体、抗鼠IgG包被受体磁珠、链亲和素供体磁珠、检测缓冲液、白色反应板、封口膜、操作说明书。其中抗鼠IgG包被受体磁珠包括100ug/mL受体磁珠，0.05%的Proclin-300和PH7.4的PBS溶液，链亲和素供体磁珠包括100ug/mL供体磁珠，25mM的HEPES、100mM的NaCl溶液和0.05%的Proclin-300。使用本发明的试剂盒建立了牛奶制品中青霉素酶的AlphaLISA检测方法，使具有样品用量少、灵敏度高、不需洗涤、检测周期短等优点。
摘要（英）：

The invention provides an AlphaLISA detection kit of penicillinase in a milk product, and the AlphaLISA detection kit comprises a penicillinase standard product, biotin penicillinase, anti-penicillinase monoclonal antibody, an anti-mouse-IgG (immunoglobulin G) coated receptor magnetic bead, a streptavidin donor magnetic bead, a detection buffer solution, a white reaction plate, a sealing membrane and an operating specification. The anti-mouse-IgG coated receptor magnetic bead includes 100 mug / mL of a receptor magnetic bead, 0.05% of Proclin-300, and a phosphate buffer solution (PBS) with the PH of 7.4, and the streptavidin donor magnetic bead includes 100 mug / mL of a donor bead, 25mM of HEPES, 100mM of a NaCl solution and 0.05% of the Proclin-300. An AlphaLISA detection method of the penicillinase in the milk product is established by use of the AlphaLISA detection kit, and the method has the advantages of less sample use amount, high sensitivity, no washing, short detecting period, and the like.

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