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发明公开
CN103543240A 发酵液中黄腐酸含量的测定方法 有权

发酵液中黄腐酸含量的测定方法
摘要：
本发明发酵液中黄腐酸含量的测定方法，该方法包括：1）发酵液中糖的测定；2）发酵液中总黄腐酸的测定；3）发酵液中纯黄腐酸含量的计算：①根据葡萄糖为与黄腐酸对应的线性关系，计算葡萄糖相当于黄腐酸的量；②以葡萄糖质量为横坐标X，对应黄腐酸质量为纵坐标Y作图得到工作曲线和线性回归方程为Y=aX+b,其中a为斜率，b为截距，将样品中的含糖量折合成黄腐酸的量，再用总黄腐酸量减去糖折合成黄腐酸的量比发酵液样品的总量，即为发酵液中纯黄腐酸的百分含量。采用本发明方法能准确测定发酵液中的黄腐酸的含量。
摘要（英）：

The invention discloses a determination method of content of fulvic acid in fermentation liquor. The method comprises the following steps of 1) determining sugar in the fermentation liquor; and 2) determining the total fulvic acid in the fermentation liquor; 3) calculating the content of pure fulvic acid in the fermentation liquor: a, calculating the content of glucose equivalent to fulvic acid according to the linear relationship that glucose is corresponding to fulvic acid; and b, mapping by taking the glucose mass as an x-coordinate and the corresponding fulvic acid mass as a y-coordinate, thereby obtaining a working curve and an equation of linear regression as Y=aX+b, wherein a refers to slope, and b refers to intercept, converting the content of sugar in a sample into the content of fulvic acid, and then subtracting the content of sugar which is converted into fulvic acid by the content of the total fulvic acid, and then dividing the total amount of the fermentation liquor sample, thereby obtaining the percentage composition of pure fulvic acid in the fermentation liquor. The method provided by the invention can accurately determine the content of fulvic acid in the fermentation liquor.

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