The invention discloses a seed guide device for an on-film hole-leveling direct sowing machine, wherein a seed diversion groove with ring distribution and for unidirectional diversion is arranged on the internal side of a roller of the device. The device further has a row spacing adjusting device, a ring cover arranged between a ring separator plate and a rotating shaft is installed on the rotating shaft by duplex bearings, and a split diversion groove of a left segment of the seed diversion groove and a left end cover of the ring cover are made of transparent material. The seed guide device has advantages that: seeds enters the bottom of the sowing machine for sowing 360 degree in advance, and operation time of the seeds is saved, thereby at least double the sowing speed, and substantially raising the sowing precision. The seed guide device can prevent the seeds generation of splashing and sowing leaking phenomena. A seed sowing rate is 100%, and a cavity phenomenon is thoroughly eliminated.