The invention provides a copolyester thermoplastic material used for three-dimensional printing and preparation and application thereof. The copolyester thermoplastic material is mainly prepared from monomers of the raw materials consisting of, by mass, 35 to 48 parts of dicarboxylic acid, 30 to 43 parts of dihydric alcohol, 8 to 15 parts of acrylate and 7 to 15 parts of a spiro-compound through copolymerization in the presence of a catalyst. The invention has the following main beneficial effects: the copolyester high-molecular material prepared in the invention has good mechanical properties and machinability, is a good thermoplastic material and has a wide application scope; high temperature resistance and moisture resistance of the material are substantially improved compared with those of traditional products, and the material has good weatherability; the material has small shrinkage, adhesion among different layers is strong, a product manufactured from the material does not suffer interlayer stripping and warping, so the material is an ideal three-dimensional printing material and is especially applicable to fused deposition modeling (FDM) technology.