The invention discloses livestock chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method.. The kit is designed with two primers and one probe in one region according to the MOMP on target sequences, and can specially amplify livestock chlamydia. The kit comprises an amplification reaction solution, a positive control, a negative control and sterilized deionized water. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time.