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发明公开
CN103571944A 芥末成分的环介导等温扩增引物、试剂盒及其应用 无效 - 驳回

芥末成分的环介导等温扩增引物、试剂盒及其应用
摘要：
本发明公开芥末成分环介导等温扩增（LAMP）引物、试剂盒及检测方法，其碱基序列如SEQ ID NO.19～24；采用LAMP检测技术，建立了适用于食品中芥末致敏原特异性检测的方法，本发明所述方法操作简单，不依赖昂贵仪器，检测时间在1小时左右，方法灵敏度达到0.5%，检测效率达到传统PCR方法或荧光定量PCR方法，能够应用于检验检疫实际工作，尤其适用于大量样品快速初筛和各种基层实验室食品品质监控，适合作为行业推荐性标准应用推广。
摘要（英）：

The invention discloses a loop-mediated isothermal amplification (LAMP) primer of a mustard component as well as a kit and a detection method thereof. Base sequences of the loop-mediated isothermal amplification primer are as shown in SEQ ID NO.19-24. An LAMP detecting technology is adopted to establish a method applicable to detecting specificity of mustard sensitinogen in food. The method disclosed by the invention is simple to operate and independent of expensive equipment, has detection time of about 1 hour, sensitivity reaching 0.5%, detecting efficiency reaching that of a conventional PCR (Polymerase Chain Reaction) method or a fluorescent quantitative PCR method, and can be applied to actual inspection and quarantine work, is especially suitable for quick preliminary screening of massive samples and monitoring quality of various base laboratory foods, and suitable for being applied and popularized as industry recommended standards.

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