The invention discloses a manual and pneumatic operating methods applicable rapid closing cut-off valve. The manual and pneumatic operating methods applicable rapid closing cut-off valve is formed by a cylinder assembly and a cut-off valve assembly; a manual translation operating mechanism is added to the left end of the cylinder assembly; the manual translation operating mechanism is formed by a small hand wheel, a cylinder pin, a manual operating lever and a limit pin. According to the manual and pneumatic operating methods applicable rapid closing cut-off valve, the problem that the rapid closing of the existing pneumatic rapid closing cut-off valve is affected by an abnormal power medium in the cylinder is solved, the structure is simple, the manufacture is easy, the rapid closing operation can be performed on the cut-off valve by manual and pneumatic operating methods which are suitable for the pneumatic rapid closing cut-off valve.