The invention discloses a method and a system for transmitting responses to access requests of a Web server. The method comprises the steps of receiving the access requests of Web application program deployed on the Web server, wherein the responses corresponding to the access requests are compiled by using scripts; transmitting testing scripts; receiving the response time of each testing script, wherein the response time includes the operation time of each testing script in a client-side browser and the network transmission time of each testing script; transmitting different responses to different types of scripts included in the responses corresponding to the access requests according to the response times of the testing scripts. The method and the system can reasonably distribute data flows between the Web browser and the Web server and enable a lot of types of client-sides to be capable of accessing the server.