The invention relates to an image positioning method of a banknote printing plate. The image positioning method comprises the steps of positioning template establishment, image splicing and image positioning. The steps are carried out in a circulation mode until all frame images of an image to be detected and an image of the positioning template are positioned. The image positioning method solves a problem that an acquired image cannot be precisely positioned relative to the template image because of image translation, rotation, scaling and the like caused by uncertain factors such as mechanical error, printing plate deformation and the like in the process of two-dimensional scanning and shooting of a camera through an image precise positioning algorithm with multiple positioning cores, and has the advantages of high positioning speed, high efficiency and high accuracy.