The invention discloses a determination method for an experiment of simulating cigarette burning for generating hydrogen cyanide. The determination method is characterized by comprising the steps of trapping hydrogen cyanide in pyrolysis products by using a Cambridge filter and absorption liquid by adopting a rapid heating control device and taking a nitrogen-oxygen mixed gas or nitrogen as a carrier gas, and then determining the HCN (hydrogen cyanide) content in the Cambridge filter and the absorption liquid by using an ion chromatography so as to realize the simulation of the cigarette burning for generating the HCN and quantitative analysis. Compared with previous methods of simulating the cigarette burning for generating the HCN, the determination method has the advantages of being large in sample loading capacity, capable of using the oxygen-nitrogen mixed gas with different ratios, high in heating rate, good in experimental repeatability and the like, without preparing experimental cigarettes, and can be applicable to the research on the rapid determination of HCN precursors in smoke.