The invention provides a detection method of emigration amount of phthalic acid ester in a textile in a human body fluid. The method is characterized by comprising the following specific steps: S1, preparing a human body fluid simulation liquid, cutting a textile sample into pieces in a reactor, adding the human body fluid simulation liquid and soaking at constant temperature; and S2, separating the textile sample from the soaking liquid, adding normal hexane into the separated soaking liquid, oscillating on a vertical oscillation instrument and carrying out liquid-liquid extraction; and carrying out nitrogen-blowing concentration on the upper extraction liquid, making up to volume by normal hexane, and detecting by a gas phase chromatographic instrument or a gas chromatography-mass spectrometer to obtain the emigration amount of the phthalic acid ester. The detection method provided by the invention has a preferable effect in acidic sweat, alkaline sweat and saliva simulation liquids.