The invention provides an MS (Mass Spectrometry) detection method for tortoise-derived ingredients in glue type traditional Chinese medicines and products thereof. The method provided by the invention comprises the step of detecting by using the difference of a specific nucleotide sequence or amino acid sequence in a genome of a tortoise, wherein the specific amino acid sequence is shown in SEQ. ID No1. By adopting the method provided by the invention, whether the glue type traditional Chinese medicines contain the tortoise-derived ingredients or not can be detected rapidly, so as to distinguish the authenticity; although polypeptides of collagen in the glue type traditional Chinese medicines are subjected to certain hydrolysis and destruction, the tortoise-derived ingredients in the glue type traditional Chinese medicines and products thereof can be identified on the basis that differential polypeptides in a main ingredient, namely collagen, of the glue type traditional Chinese medicines are high in content and little in damaged extent.