A sheep placenta not only has rich nutritional value, but also has a cell immune function, a cell repairing function and the like, but active ingredients in the sheep placenta have significant change after the sheep placenta is separated from a maternal body and show a significantly decreasing tendency. The invention discloses a method for on-site collection and fresh preservation of the sheep placenta and an amniotic fluid. The method comprises the steps: preparing double interlayer collection bags each having an outer layer which is a cold medium; respectively filling the amniotic fluid and the sheep placenta into the collection bags for vacuum package; putting into an instant freezer for ultrarapid freezing, and carrying out low-temperature frozen storage; and thawing the frozen-storage amniotic fluid and the frozen-storage sheep placenta by running water or microwaves for follow-up development. The method prevents the change of the active ingredients of the amniotic fluid and the sheep placenta during the collection process, maximizes to maintain nutrient substances and the active ingredients of the amniotic fluid and the sheep placenta, and provides a basic guarantee for deep processing of the amniotic fluid and the sheep placenta. The method has short quick-freezing time, simple process and convenient operation, and is a new technology for retaining the maximum bioactive ingredients of the amniotic fluid and the sheep placenta.