The invention discloses an in vitro micro-dynamic culture device of mammalian embryos and a method for cultivating embryos by using the same, relates to an in vitro culture device of embryos and a cultivation method using the same, and overcomes the defects of an in vitro oscillating micro-dynamic incubator of mammalian embryos and the defects of an existing patent technology. The culture device comprises a glass micro-fluid channel layer, a main body layer, an air chamber seal cover, an air duct, an injector, an electric motor and a square wave generator. The cultivation method comprises the following steps: 1, setting the frequency of the square wave generator and the stretching speed of the electric motor; 2, injecting a culture solution and mineral oil, and putting the embryos into a culture room; and 3, powering on a switch, and cultivating. The in vitro micro-dynamic culture device is applied to the field of biology.