A diagnosis kit for rapidly determining the sensitivity of Aphis citricola to bifenthrin is formed by a glass tube, a sensitive layer and a cotton plug. The sensitive layer is obtained through a step of uniformly smearing the inner wall and the bottom of a finger-shaped tube with 500muL of an acetone solution of bifenthrin having a concentration of 1.3/L and a step of rolling air-drying, and the air-dried finger-shaped tube is plugged with the cotton plug to form the diagnosis kit. The use method of the kit comprises the following steps: carrying the prepared diagnosis kit to the field, opening the cotton plug, lightly sweeping 20 Aphis citricola having a same size into the finger-shaped tube, plugging with the cotton plug, and inspecting the mortality 60min later. The Aphis citricola mortality is used to determine the sensitivity of the Aphis citricola to bifenthrin, and the kind and the amount of applied pesticides are determined from the sensitivity. The diagnosis kit allows the sensitivity of the Aphis citricola to bifenthrin to be rapidly and simply determined within 2h, and provides a scientific basis for the scientific and effective guidance of the field Aphis citricola control.