The invention relates to a cigarette holder antibacterial layer of a low-temperature cigarette. The invention relates to the field of low-temperature cigarettes, and particularly relates to preparation and applications of the cigarette holder antibacterial layer of the low-temperature cigarette. The antibacterial layer is an antibacterial membrane material obtained by composition of an adsorption substrate with antibacterial functions and an antibacterial agent. The antibacterial layer is adhered to the cigarette holder of the low-temperature cigarette. The antibacterial layer is simple in technology, reduces harm caused by bacterial growth to a customer during a burning and smoking process of the cigarette to some extent, and provides and maintains sanitary smoking environment for customers.