The invention discloses organic detritus cenobium bait for hypophthalmichthys nobilis and a preparation method of the bait, and relates to the technical field of fishing feeds. The bait comprises the following components in percentage by weight: 45-75% of fermented chicken manure, 9-21% of fermented rapeseed meal, 2-8% of chlorella powder, 2-8% of brewer grain, 0.6-1.4% of algae growth prompting microelements, 2-6% of a phagostimulant and 4-16% of a premix. The preparation method of the product comprises the following steps: fermenting, drying and crushing the chicken manure and the rapeseed meal, and mixing the prepared fermented chicken manure, fermented rapeseed meal and other components so as to obtain the bait. The invention aims at providing the organic detritus cenobium bait for the hypophthalmichthys nobilis and the preparation method of the bait, and by adopting the bait, not only is the acre yield of the hypophthalmichthys nobilis increased, but also the secondary pollution on aquatic water is reduced.