The present invention provides a composition for promoting erythropoiesis and uses thereof. The composition comprises two or more flavonoid compounds as active ingredients. The flavonoid compound is represented by formula I. R1 is hydroxy or (II); R2 is H or hydroxy. In preparing the composition, the flavonoid compound is used in a purified form. The composition of the invention has good synergistic effects on enhancing the transcriptional activity of HRE to promote erythropoiesis and preventing and treating anemia and/or anoxia, significantly reducing the amount of each component, and has low prices and toxic side effects.