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发明公开
CN103695528A 一种定量检测食烷烃戈登氏菌的特异性引物 无效 - 撤回

一种定量检测食烷烃戈登氏菌的特异性引物
摘要：
本发明公开了一种定量检测食烷烃戈登氏菌的特异性引物，属于环境微生物技术领域，引物由SEQ ID NO.1所示的上游引物和由SEQ ID NO.2所示的下游引物组成。通过环境样品DNA提取、定量PCR检测、PCR产物分析等步骤，采用本发明提供的特异性引物可以进行食烷烃戈登氏菌的定量检测。本发明具有特异性高、灵敏度高和检测速度快耗时短等优点。
摘要（英）：

The invention discloses a specific primer for quantitative determination of gordonia alkanivorans, and belongs to the technical field of environmental microorganisms. The primer comprises an upstream primer as shown in SEQ ID NO. 1 and a downstream primer as shown in SEQ ID NO. 2. Through steps of environmental sample DNA extraction, quantitative PCR detection, PCR product analysis and the like, the specific primer provided in the invention can be used for quantitative determination of gordonia alkanivorans. The specific primer of the invention has the advantages of high specificity, high sensitivity, high detection speed, and low time consumption.

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