A fabric anti-mite finishing agent comprises the following components in parts by mass: 4-12 parts of sophora flavescens, 5-8 parts of yellow lead oxide, 8-12 parts of thymol, 2-5 parts of an emulsifier, 1-3 parts of diepoxy triglycol glycidyl ether, 8-10 parts of silk fibroin, and 30-50 parts of water. Preferably, the fabric anti-mite finishing agent comprises the following components in parts by mass: 8 parts of sophora flavescens, 6 parts of yellow lead oxide, 10 parts of thymol, 4 parts of the emulsifier, 2 parts of diepoxy triglycol glycidyl ether, 9 parts of silk fibroin, and 40 parts of water. The emulsifier is triethanolamine or sodium lauryl sulfate. The fabric anti-mite finishing agent provided by the invention has safe components, adopts natural bacteriocidal substance formula, allows a finished fabric to have relatively strong anti-mite and bacteriocidal abilities, is safe, and has no residues. The fabric after being finished by the finishing agent has the mite avoidance rate of up to 90% or more, and still has the mite avoidance rate of up to 85% or more after being soaked in water for half an hour.