The invention provides a multi-flower paphiopedilum test-tube seedling cultivation method, belonging to the technical field of plant growing. The method is characterized by comprising the following steps: 1) pod acquisition; 2) aseptic seeding; 3) multiplication subculture; 4) root and strong seedling culture; and 5) test-tube seedling transplantation. The multi-flower paphiopedilum test-tube seedling cultivation method has the characteristics of high germination rate, short seedling emergence cycle, robust seedlings, developed root systems, good seedling quality, low production cost and the like, can be used for large-scale industrialized production of paphiopedilum and has a good application prospect.