The invention belongs to the technical field of inorganic materials, and particularly relates to a preparation method of a nanorod particle ordered-assembly ZSM-5 zeolite molecular sieve. By adopting a hydrothermal synthesis method, a silicon source, an aluminum source, an alkali source, a MFI zeolite crystal seed, an inorganic salt and a water phase are mixed and stirred into a gel mixture which is used as a raw material for synthesizing the ZSM-5 zeolite molecular sieve. The chemical composition of the MOR zeolite in such special structure is TO2:aY2O3:bM2/nO, wherein T represents at least one quadrivalent element; Y represents at least one trivalent element, such as Al, B, Ga, Fe and the like; M represents at least one alkali metal or alkali earth metal element, and the valence state is n; a and b are respectively mole numbers of Y2O3 and M2/nO; and a is 0-0.5, and b is 0-0.5. The method has the advantages of lower cost and no environmental pollution, and is suitable for popularization.