Proposed are a method and a device for positioning a plurality of objects of interest in CT imaging. Three projected images under an approximately-vertical visual angle are used to realize three-dimensional space positioning of objects of interest. The method is capable of determining the positions of the objects of interest in a CT image rapidly under a condition that a plurality of objects of interest exist in a scanning vision and does not require pre-reconstruction of the CT image and an algorithm does not relate to iterative steps so that the method is rapid and effective and well applicable to engineering application.