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CN103717751A 用于多重核酸鉴定的产品和方法 无效 - 驳回 转让 复审申请

用于多重核酸鉴定的产品和方法
摘要：
本文提供了用于检测多重靶核酸是否存在的产品和方法。某些方法包括扩增所述靶核酸或其部分；延伸与扩增子特异性杂交的寡核苷酸，其中所述延伸的寡核苷酸包括捕获剂；通过所述捕获剂将所述延伸的寡核苷酸捕获至固相；通过用竞争物的竞争释放所述延伸的寡核苷酸；检测所述延伸的寡核苷酸，并且从而通过所述延伸的寡核苷酸是否存在确定每种靶核酸是否存在。
摘要（英）：

Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.

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