The invention discloses a fluorescence analysis method (/b) for determining glucose by employing (b) nanometer copper oxide as simulated peroxide. The fluorescence analysis method for determining glucose by employing nanometer copper oxide as simulated peroxide is characterized by comprising the following steps: firstly, mixing glucose, glucose oxidase and phosphate buffer in a warm bath, and then adding the phosphate buffer; continuing to carry out warm bath on terephthalic acid and the nanometer copper oxide, wherein the fluorescence excitation wavelength and the emission wavelength of a reaction product of the mixing solution are 315nm and 421nm, the linear range of glucose content measurement is 3-120mumol/L, and the detection limit is 1.94mumol/L. Therefore, the method can be applied to determination of blood sugar concentration.