The invention discloses a method for batch breeding of queen bees or fixed-day-age worker bees. According to the method, the queen bees or the fixed-day-age worker bees can be bred in batches by adopting a special device. The device is provided with queen bee breeding boxes and a bee nest frame, wherein the bee nest frame is divided into six nest grids by horizontal partition plates and vertical partition plates; the queen bee breeding boxes are placed in the nest grids and are fixed by clamping strips; each queen bee breeding box is provided with five breeding chambers; the breeding chambers can be used for breeding the queen bees, and can also be used for breeding the worker bees; the breeding chambers are mutually independent to facilitate marking; 30 queen bees can be bred in each device; 10 to 20 worker bees are placed in each breeding chamber; 300 to 600 worker bees can be bred by the device. Therefore, the method solves the difficulty of the batch breeding of the queen bees or the fixed-day-age worker bees in the same bee colony, and has great significance on accelerating bee breeding in early spring, storing purebred systems of the bees, ensuring the normal research work and ensuring smooth completion of the queen bee production plan and the like.