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发明公开
CN103736079A 含有EGF的植物油体凝胶剂 无效 - 驳回

含有EGF的植物油体凝胶剂
摘要：
本发明提供了含有EGF的植物油体凝胶剂，它是由含有EGF的植物油体和油体的凝胶基质混合制得，所述的油体的凝胶基质，是由羧乙基纤维素1%—5%、甘油5%—40%、尼泊金甲酯0.01%—0.1%、尼泊金丙酯0.01%—0.1%、加注射用水至100%；含有EGF的植物油体，是将人EGF的核苷酸序列连接到油体表达载体p1390ONE的油体蛋白核苷酸序列的3’端，转入油料作物油体中表达了EGF，提取的油体，制成的植物油体凝胶剂，37℃保存12个月，凝胶剂无霉变现象，其外观、pH等理化指标均未发生变化；4℃保存12个月，EGF活性无明显变化。
摘要（英）：

The invention provides a vegetable oil body gel containing EGF (Epidermal Growth Factor). The vegetable oil body gel is obtained by mixing a vegetable oil body containing the EGF and a gel matrix of the oil body, wherein the gel matrix of the oil body is composed of 1%-5% of carboxyethyl cellulose, 5%-40% of glycerol, 0.01%-0.1% of methyl paraben, 0.01%-0.1% of propyl paraben, and water injection added to 100%. The vegetable oil body containing the EGF is obtained through the steps of connecting the nucleotide sequence of human EGF to the terminal 3' of the oil body protein nucleotide sequence of the oil body expression vector p1390ONE, transferring to the oil plant so that the EGF is expressed in the oil body, and then extracting the oil body. The prepared vegetable oil body gel is free of mildew and the physicochemical indexes such as appearance and pH of the vegetable oil body gel all are not changed after the vegetable oil body gel is preserved for 12 months at 37 DEG C; even though the vegetable oil body gel is preserved for 12 months at 4 DEG C, the activity of the EGF has no obvious change.

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