The invention discloses a culture medium for culturing peanut gynophores, which is prepared from a seedling raising substrate used as a main raw material, and white lime and a gelatinizer used as auxiliary materials. The culture medium is prepared by evenly mixing the white lime, gelatinizer and seedling raising substrate in a mass ratio of 1:(5-380):(25-1520). The culture medium mainly solves the problem that the overground gynophores of peanuts can not penetrate into soil to develop into full pods, enhances the propagation coefficients of peanut hybrid F1 and rare seed resources, and can solve the problem that the interspecific cross subsequent generation can not form pods since the plant grows vigorously and the gynophores are too far away from the ground.