利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN103740822B 一种SPF小鼠病原菌核酸荧光定量PCR联合快速检测试剂盒及检测方法 有权

一种SPF小鼠病原菌核酸荧光定量PCR联合快速检测试剂盒及检测方法
摘要：
本发明涉及一种SPF小鼠病原菌核酸荧光定量PCR联合快速检测试剂盒及检测方法。目的是提供的检测试剂盒应可同时对四种常见SPF动物携带病原进行检测；提供的方法具有操作简单、检测快速的特点。技术方案是：一种SPF小鼠病原菌核酸荧光定量PCR联合快速检测试剂盒，包括实时荧光定量PCR预混体系以及ddH2O，该试剂盒还包括针对4种病原菌的4对特异性引物；特异性引物如下：金黄色葡萄球菌；肺炎克雷伯菌；沙门氏菌；绿脓杆菌。一种SPF小鼠病原菌核酸荧光定量PCR联合快速检测方法，按以下步骤进行：（1）提取样品DNA；（2）以样品DNA为模板，进行联合PCR检测；（3）反应产物置于定量PCR仪进行荧光检测。
摘要（英）：

The invention relates to a fluorescence quantitative PCR (polymerase chain reaction) combined rapid detection kit and a fluorescence quantitative PCR combined rapid detection method for SPF (specific pathogen free) mice pathogenic bacteria nucleic acid. The provided detection kit aims to be capable of simultaneously detecting four common SPF animal carried pathogens. The provided method has the characteristics of simplicity in operation and rapidness in detection. According to the technical scheme, the fluorescence quantitative PCR combined rapid detection kit for the SPF mice pathogenic bacteria nucleic acid comprises a real-time fluorescence quantitative PCR pre-mixing system, ddH2O and four pairs of specific primers for four pathogenic bacteria, wherein the specific primers for staphylococcus aureus, klebsiella pneumoniae, salmonella and pseudomonas aeruginosa are shown in the specification. The fluorescence quantitative PCR combined rapid detection method for the SPF mice pathogenic bacteria nucleic acid comprises the following steps: (1) extracting sample DNA (deoxyribonucleic acid); (2) by using the sample DNA as a template, performing combined PCR detection; (3) placing a reaction product in a quantitative PCR instrument for fluorescence detection.

公开/授权文献
0/0