The invention relates to a vibrio cholera analysis typing kit. A fluorescently-marked composite primer is used for amplifying six VNTR (variable number of tandem repeats) loca of vibrio cholera respectively. According to the vibrio cholera analysis typing kit provided by the invention, the six loca are divided into three groups, and four fluorescent markers are involved in total; after capillary electrophoresis detection, by comparing with an allele typing standard substance, the type of vibrio cholera can be obtained quickly and accurately.