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发明公开
CN103757138A 一种马铃薯病毒的快速灵敏检测方法 有权

一种马铃薯病毒的快速灵敏检测方法
摘要：
本发明公开了一种云南马铃薯主栽品种丽薯6号、云薯401、宣薯2号病株中马铃薯Y病毒、马铃薯X病毒、马铃薯A病毒和马铃薯卷叶病毒4种病毒的检测方法。本发明通过对云南马铃薯主栽品种丽薯6号、云薯401、宣薯2号病株中马铃薯Y病毒、马铃薯X病毒、马铃薯A病毒和马铃薯卷叶病毒4种特异性混合抗体诱捕病样中病毒，不必提取样品总RNA进行随机引物逆转录和特异性病毒引物复合PCR反应。本方法将快速、特异的免疫沉淀与灵敏的复合RT-PCR结合，同时设置健康马铃薯随机引物cDNA产物和相应病毒PCR特异片段阳性质粒作为阴、阳性对照，从而快速、准确、灵敏的应用于检测田间马铃薯植株样品、抗病毒材料及田间环境疑似侵染源等方面。
摘要（英）：

The invention discloses a method for potato Y virus, potato X virus, potato A virus and potato leaf roll virus in Yunnan potato main culture species Li potato No.6, Yun potato 401 and Xuan potato No.2 infected plants. The specific mixed antibody is utilized to entrap the potato Y virus, potato X virus, potato A virus and potato leaf roll virus in Yunnan potato main culture species Li potato No.6, Yun potato 401 and Xuan potato No.2 infected plants, so that the total RNA (ribonucleic acid) of the sample does not need to be extracted to perform random primer reverse transcription and specific virus primer compound PCR (polymerase chain reaction) reaction. The method combines quick specific immune precipitation and sensitive compound RT-PCR (reverse transcription-polymerase chain reaction), sets healthy potato random primer cDNA (complementary deoxyribonucleic acid) product and corresponding virus PCR specific segment positive plasmid as negative and positive controls, and thus, is quickly, accurately and sensitively used in detecting field potato plant samples, antiviral materials, field environment suspect infection sources and the like.

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