The invention relates to a sealing cover used for mushroom cultivation. The sealing cover comprises a hollow sleeve base, a cover body and a filter material. The hollow sleeve base is provided with a top end opening and a bottom end opening opposite to the top end opening. A cultivation tool is sleeved with the bottom end opening. The cover body covers the top end of the hollow sleeve base. A plurality of ventilation grooves are formed in the periphery of the cover body, communicated with the top end opening of the hollow sleeve base and used for providing pipelines for gas exchange. The filter material is arranged between the hollow sleeve base and the cover body, covers the top end opening of the hollow sleeve base and is used for providing the effects of water prevention, ventilation and external bacteria isolation.