The invention discloses a high-yield pollution-free breeding method of freshwater fish. The method includes the following steps that requirements for a breeding pond are met; the pond is filled with water, and water quality is adjusted; fish varieties are selected; fish varieties are sterilized; reasonable fish stocking is performed; fish varieties are bred; the water quality is controlled; after water is added into the pond every time, a certain amount of special fertilizer for aquaculture and saccharomycete are added into the breeding pond, and moderate fishing is performed. According to the method, due to sterilization and scientific breeding of the fish pond and the fish bodies, the using amount of medicine and artificial feed is effectively reduced, and the bred freshwater fishes are high in quality safety and free of pollution; due to the mixing breeding of the fish varieties living in different depths of water areas, the fishes are diversified in variety, space of the fish pond in different depths of water areas is effectively utilized, and not only is breeding number improved but also income of fishermen is increased due to orderly fishing according to batches.