The invention discloses a method for measuring the Co2 generating rate of a cultivating matter by adopting a seal dynamical system under an indoor cultivating condition, and relates to a method for measuring the Co2 generating rate of the cultivating matter. The method provided by the invention solves the problems that the accuracy rate is low, the cost is high and the labor capacity is large in an existing method. The labor capacity method comprises the following steps of 1, adding the cultivating matter into a cultivating bottle for cultivating; 2, adding the cultivating bottle in a seal dynamical system, and measuring the real-time dynamic state of a Co2 concentration; and 3, calculating the Co2 generating rate of the cultivating matter by using a linear regression method. The method provided by the invention well solves the limitation that a cultivating condition is changed when the existing method is used for measuring the Co2 generating rate of the cultivating matter under the indoor cultivating condition, the test cost is high, the human factor influence is large, the result is not exact, the Co2 generating rate of the cultivating matter is exactly rapidly cheaply measured, the application range of a Co2 analysis meter such as LI-820 is widened, and the method provided by the invention is used for measuring the Co2 generating rate of the cultivating matter.