Provided are a controller for a disassembly preventing network and a data security device. The controller comprises a PUF unit, an ROM unit and a matching unit, wherein the PUF unit is used for producing a random number; the ROM unit uses the random number as an address index so as to provide a first sequence, so that an external device and a second sequence corresponding to the first sequence are controlled; the matching unit enables the second sequence to be matched with output produced when the external device responds to control of the first sequence, and an alarming signal is output when matching is unsuccessful. The data security device comprises the disassembly preventing network and the controller, wherein the disassembly preventing network is formed by connecting switch nodes in a switch node matrix, and the disassembly preventing network can be controlled by the controller so as to be connected into different Hamilton paths. By means of the controller for the disassembly preventing network and the data security device, the disassembly preventing network can be effectively prevented from being attacked by attackers through reverse engineering, and therefore security is enhanced.