The invention provides a soil bacteriostatic activity detection method and the application thereof. The method comprises the steps that a certain amount of soil lixivium is obtained, a certain soil pathogenic bacterium is inoculated, micro-cultivation is carried out in the head of a pipette, by detecting the growth amount of pathogenic bacteria, soil bacteriostatic activity is detected, and the bacteriostatic capacity of the soil can be quantized in a macroscopic mode. Meanwhile, the detection method can be used for detecting the bacteriostatic activity of the soil in which soil additives are used, and accordingly soil additives with high soil bacteriostatic activity can be screened out.